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Identification and Optimization of qPCR Standards for Aiptasia pallida --June, 2012: (.pdf)


Table of antibodies tested by the Pringle Lab

Perez Anitbodies Table for download (.xls)

Expand: Table of Antibodies Tested by the Pringle Lab

Antibody Name
Immunogen
Applications (IHC, WB)
Successful?
Notes
Tested by
Supplier
Catalog Number
anti-histone H2A
Histone H2A (Human)
IHC of whole mounts and sections
Yes
Rabbit polyclonal; good for staining Aiptasia nuclei specifically; used at 1:500
Santiago Perez
Abcam
ab58550
anti-Histone H3 (phospho T3)
Histone H3 phosphorylated at T3 (S. cerevisiae)
IHC whole mounts and sections
Yes
Rabbit polyclonal, Stains mitotic chromatic of Aiptasia really well, very little background; used at 1:500 in IHC
Santiago Perez
Abcam
ab17352
anti-phospho-Histone H3 (Ser 10)
Histone H3 phosphorylated at Ser10 (Human)
IHC of whole mounts and sections
Yes
Rabbit polyclonal, Stains mitotic chromatic of Aiptasia very well with very little background; used at 1:500 in IHC
Santiago Perez
Millipore
06-570
anti-Ki67
Ki67 (Human)
IHC of sections
No
Rabbit monoclonal; tested to label proliferating cells; tested at 1:100; 1:500
Santiago Perez
Thermo Scientific
RM-9106
anti-YOL1/34
Tubulin (S. cerevisiae)
IHC of whole mounts, WB
Yes
Rat monoclonal; stains mitotic spindle; used at 1:500; band at 51 kD in WB
Santiago Perez, Liz Hambleton
Abcam
ab6161
anti-Actin, clone C4
Actin (Chicken)
IHC of whole mounts and sections, WB of Aiptasia protein extract
Yes
Mouse monoclonal, band at 42 kD in WB of Aiptasia protein; used at 1:500 in IHC
Santiago Perez, Liz Hambleton
Millipore
MAB1501
anti-coracle
Coracle (Drosophila)
IHC of whole mounts
No
Mouse monoclonal against a component of septate junctions, tested to delineate Aiptasia cell-perimeter (obtained from Dr. Richard G. Fehon of U. of Chicage, Dept. of Mol. Gen. and Cell Bio.);  tested at 1:50, 1:100, 1:500
Santiago Perez
Developmental Studies Hybridoma Bank

C566.9

and C615.16

anti-E-Cadherin
epithelial cadherin (Mouse)
IHC of whole mounts
No
Rat monoclonal; tested as a marker of cell perimeter (usuccessfully); tested at 1:50, 1:100, 1:500
Santiago Perez
Zymed (Invitrogen)
13-1800
anti-LC3
Residues 25-121of the human Microtuble-associated protein light chain 3 (LC3) protein sequence
WB
Yes
Rabbit polyclonal, band at 14 kD in WB of Aiptasia protein
Tamaki Bieri
Novus Biologicals
NB100-2331
gp62ap
recombinant portion of Aiptasia C3
IHC of whole mounts
Yes
Custom antibody, guinea pig polyclonal, affinity purified by Liz Hambleton; successful in whole mount IHC
Liz Hambleton
Cocalico Biologicals
-
gp64ap
recombinant portion of Aiptasia C3
IHC of whole mounts
Yes
Custom antibody, guinea pig polyclonal, affinity purified by Liz Hambleton; successful in whole mount IHC
Liz Hambleton
Cocalico Biologicals
-
gp65ap
recombinant portion of Aiptasia C3
IHC of whole mounts
Yes
Custom antibody, guinea pig polyclonal, affinity purified by Liz Hambleton; successful in whole mount IHC
Liz Hambleton
Cocalico Biologicals
-
anti-C3 
internal portion of human C3
WB
No
Goat polyclonal; successful in WB of mouse serum, but not with Aiptasia protein extract
Liz Hambleton
Santa Cruz Biotech
sc-14613