Summaries of lab projects: |
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We assembled the transcriptome of a clonal population of adult, aposymbiotic (dinoflagelate-free) Aiptasia pallida from ~208 million reads, yielding 58,018 contigs. We demonstrated that many of these contigs represent full-length or near-full-length transcripts that encode proteins similar to those from a diverse array of pathways in other organisms, including various metabolic enzymes, cytoskeletal proteins, and neuropeptide precursors. |
The contigs were annotated by sequence similarity, assigned GO terms, and scanned for conserved protein domains. |
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| Many alternatively spliced transcript were present in out transcriptome. For the purposes of RNA-Seq experiments, transcripts with highly redundant sequence should be removed. Below is a fasta file of containing the longest transcript from each cluster. Adult aposymbiotic Aiptasia transcriptome FOR MAPPING version 1.1 |
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Transcriptome of Symbiotic CC7 Aiptasia (with some algal sequences as well): Any transcripts having 99% identity over an alignment that covers at least 20% of both transcripts were clustered and a single representative transcript retained in this GZ file. |