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Micrographs of Yeast bud neck

Ryuichi's micrographs make the cover:


Progression of cytokinesis in S. cerevisiae
Electron micrographs of yeast bud necks

(Top) Wild type; a thin cleavage furrow containing chitinous primary septum (white) ingresses and is later sandwiched by secondary septa (grey).

(Bottom) inn1-delta mutant; a wide furrow containing secondary-septum material ingresses without a primary septum, indicating the essential role for Inn1p in primary-septum formation.

Time-lapse analysis of Inn1p-GFP

Inn1p-GFP (top left), Cdc3p-CFP (top right), and DIC (bottom left). Frames were collected every minute and shown at 6 frames/sec. The Inn1p ring is formed concurrently with the spitting of the septin hourglass (Cdc3p), indicative of exit from mitosis. For more stories and related movies, please visit the journal web site: Journal of Cell Biology: Nishihama / Onishi

Budding scar patterns Budding-pattern phenotypes

Calcofluor staining marks the previous division sites. We refer to these as bud scars.

A) Wild-type haploid

B) Wild-type diploid

C) ste20-delta diploid

D) bud7-delta diploid
Mitosis figures